Alkaline Protease Associated with Virus Particles of a Nuclear Polyhedrosis Virus: Assay, Purification, and Properties
نویسندگان
چکیده
منابع مشابه
Characterization of nuclear polyhedrosis virus DNAs.
The nuclear polyhedrosis virus DNAs characterized and compared in this study consist of the singly-enveloped nucleocapsids (SNPV) of Trichoplusia ni and the bundles of nucleocapsids common to a single envelope (MNPV) from Spodoptera frugiperda and Rachiplusia ou. The SNPV and MNPV DNAs are very similar in hydrodynamic properties and molecular weights. In addition, the NPV DNAs are similar in si...
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There have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. While there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. Three possible ways have...
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Nonoccluded virus, polyhedra, and occluded virus were purified from a continuous cell culture of Spodopera frugiperda infected with nuclear polyhedrosis virus. The optimal temperature for the replication and lateral transmission of infectivity for the nuclear polyhedrosis viruses (NPV) in cell culture was 27 C. End-point dilution and plaque assay procedures for the measurement of infectivity ar...
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The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
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The indiscriminate use of pesticides for the last 40 years has almost eliminated natural enemies from many crop eco-systems. This scenario has led many countries to consider the potential of biological control as a component of pest management. At present, the world market for microbial pesticides is in excess of US $ 125 million per annum which is still less than 1 percent of the total global ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1978
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.26.1.84-92.1978